Browsing by Author "Dias, MF"
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- Atipias glandulares de significado indeterminado (AGUS) que entidade ?Publication . Pires, MA; Rebelo, MT; Dias, MF; Amaral, N; Dinis, M; Oliveira, CF; Oliveira, HM
- Avaliação da eficácia terapêutica do ceftriaxone versus piperacilina na profilaxia da infecção pós-histerectomiaPublication . Paulo, AS; Marta, J; Guerra, C; Tavares, M; Leitão, F; Riscado, I; Raimundo, D; Dias, MF; Ngan-Va, L; Oliveira, CF
- Breast cancer patients with micrometastases in sentinel lymph nodes: differences considering additional metastatic lymph nodesPublication . Carvalho, MJ; Dias, MF; Silva, T; Custódio, S; Oliveira, CF
- Chemoprevention of DMBA-Induced Mammary Tumors in Rats by a Combined Regimen of Alpha-Tocopherol, Selenium, and Ascorbic AcidPublication . Dias, MF; Sousa, E; Cabrita, S; Patrício, J; Oliveira, CFThis experimental study was designed to evaluate the efficacy of associated naturally occuring antioxidants in the prevention of chemically induced breast cancer using DMBA in virgin female Wistar rats. Rats were randomly allocated to three groups: control group (CG; n = 20), induction group (IG; n = 100), and prevention group (PG; n = 70). A single dose (65 mg/kg) of DMBA was administered in the IG and PG animals at 50 days of age. PG animals also received a single dose of alpha-tocopherol (200 mg/rat) 1 hour after DMBA administration and an association of selenium (p-XSC, 40 ppm/day/rat) and ascorbic acid (540 mg/day/rat) in drinking water, daily, from carcinogenic induction until necropsy. Macroscopic study and pathology revealed a significantly lower development of neoplasms in the PG animals (p < 0.05); the number of rats with mammary tumors, breast cancer incidence, and the number of malignant breast tumors per rat as well as per tumor-bearing rat were significantly decreased in the PG animals. Other types of primary neoplasms existing in the IG animals totally disappeared in the PG animals. Immunostaining to hormone steroid receptors (ER and PR) and cathepsin D was similar in both groups. Overexpression of p53 and metallothioneine was significantly increased in the PG animals (p < 0.05) and immunostaining to bromodeoxiuridin and Ki-67 was also stronger in the remaining tumors in the PG animals. These data thus add to the accumulating evidence that those micronutrients in combination seem to be effective in reducing the incidence of malignant tumors. Nevertheless, remaining tumors seem to present more aggressive behavior and characteristics of drug resistance
- Évaluation des 3400 hystérectomies par voie abdominale réalisées dans le service de gynécologie des hôpitaux de l´Université de CoimbraPublication . Dias, MF; Lobo, AC; Oliveira, CF; Almeida-Santos, A; Oliveira, HM
- Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patientsPublication . Silva, HA; Abraúl, E; Raimundo, D; Dias, MF; Marques, C; Guerra, C; Oliveira, CF; Regateiro, FJ;The aim of this study is to evaluate epidermal growth factor receptor variant III, EGFRvIII, a cancer specific mutant, as a possible marker for the diagnosis of breast cancer occult systemic disease. EGFRvIII mRNA was identified by an RT-nested PCR with a high sensitivity. In 102 women studied, the mutant was detected in the peripheral blood of 30% of 33 low risk, early stage patients, in 56% of 18 patients selected for neoadjuvant chemotherapy, in 63.6% of 11 patients with disseminated disease and 0% of 40 control women. In low risk, early stage patients, the presence of one or more tumour characteristics predicting recurrence such as the absence of oestrogen receptors and the presence of ERBB2 or histologic grades G2/G3 was significantly associated with EFGRvIII detection (p<0.05). EGFRvIII mRNA has characteristics to be a useful marker for the diagnosis of occult systemic disease in breast cancer. Follow-up studies will evaluate its clinical value as a decision criterion for systemic therapy.
- Serologic profile of some sexually transmitted diseases in women with squamous intraepithelial lesionsPublication . Gomes, C; Dias, MF; Falcão, F; Oliveira, CFThe purpose of this study consisted of the evaluation of some sexually transmitted diseases in patients with cervical pathology, namely squamous intraepithelial lesions. METHODS: a prospective study was performed. Patients with an abnormal cervical smear were submitted to colposcopy, directed biopsy and an immunologic assay for Chlamydia, Herpes Simplex Virus (HSV) types 1 and 2, Cytomegalovirus, Treponema pallidum, Hepatitis B and Human Immunodeficiency Virus I and II. The same parameters were evaluated in women with normal cervical cytology in a matched control group. A comparative study was performed evaluating some epidemiological parameters and the referred immunologic assays. RESULTS: 118 patients were separated into four groups. Statistically significant differences were observed in the personal history of fungi infections, as well as Chlamydia and HSV 2 IgM. CONCLUSION: immunologic assays may prove useful in identifying sexually-transmitted diseases, especially Chlamydia and HSV 2 infections, in Human Papillomavirus infected women.