Publication
Release of IL-1beta via IL-1beta-converting enzyme in a skin dendritic cell line exposed to 2,4-dinitrofluorobenzene
| dc.contributor.author | Matos, MT | |
| dc.contributor.author | Jaleco, SP | |
| dc.contributor.author | Gonçalo, Margarida | |
| dc.contributor.author | Duarte, CB | |
| dc.contributor.author | Lopes, MC | |
| dc.date.accessioned | 2010-03-10T15:18:46Z | |
| dc.date.available | 2010-03-10T15:18:46Z | |
| dc.date.issued | 2005 | |
| dc.description.abstract | We used a mouse fetal skin dendritic cell line (FSDC) to study the effect of the strong allergen 2,4-dinitrofluorobenzene (DNFB) on interleukin (IL)-1beta release and IL-1beta receptor immunoreactivity. Stimulation with DNFB (30 minutes) increased IL-1 release without changing the mRNA levels of the protein. Furthermore, DNFB increased transiently the interleukin-1beta-converting enzyme (ICE) activity, as measured with its fluorogenic substrate Z-Tyr-Val-Ala-Asp-AFC. The ICE inhibitor Z-YVAD-FMK prevented the release of IL-1beta evoked by DNFB. Incubation of the cells with DNFB (30 minutes) strongly increased IL-1beta receptor immunoreactivity. The rapid effect of DNFB on the release of mature IL-1beta, without inducing an increase of IL-1beta mRNA in FSDC, suggests a posttranslational modification of pro-IL-1beta by ICE activity. | pt |
| dc.identifier.citation | Mediators Inflamm. 2005 Aug 14;2005(3):131-8. | pt |
| dc.identifier.uri | http://hdl.handle.net/10400.4/755 | |
| dc.language.iso | eng | pt |
| dc.publisher | Hindawi Publishing Corporation | pt |
| dc.subject | Células Dendríticas | pt |
| dc.subject | Dinitrofluorobenzeno | pt |
| dc.subject | Pele | pt |
| dc.title | Release of IL-1beta via IL-1beta-converting enzyme in a skin dendritic cell line exposed to 2,4-dinitrofluorobenzene | pt |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | pt |
| rcaap.type | article | pt |