Publication
Evaluation of the anti-inflammatory, anti-catabolic and pro-anabolic effects of E-caryophyllene, myrcene and limonene in a cell model of osteoarthritis
| dc.contributor.author | Rufino, AT | |
| dc.contributor.author | Ribeiro, M | |
| dc.contributor.author | Sousa, C | |
| dc.contributor.author | Judas, F | |
| dc.contributor.author | Salgueiro, L | |
| dc.contributor.author | Cavaleiro, C | |
| dc.contributor.author | Mendes, AF | |
| dc.date.accessioned | 2015-02-10T10:02:37Z | |
| dc.date.available | 2015-02-10T10:02:37Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | Osteoarthritis is a progressive joint disease and a major cause of disability for which no curative therapies are yet available. To identify compounds with potential anti-osteoarthritic properties, in this study, we screened one sesquiterpene, E-caryophyllene, and two monoterpenes, myrcene and limonene, hydrocarbon compounds for anti-inflammatory, anti-catabolic and pro-anabolic activities in human chondrocytes. At non-cytotoxic concentrations, myrcene and limonene inhibited IL-1β-induced nitric oxide production (IC50=37.3μg/ml and 85.3µg/ml, respectively), but E-caryophyllene was inactive. Myrcene, and limonene to a lesser extent, also decreased IL-1β-induced NF-κB, JNK and p38 activation and the expression of inflammatory (iNOS) and catabolic (MMP-1 and MMP-13) genes, while increasing the expression of anti-catabolic genes (TIMP-1 and -3 by myrcene and TIMP-1 by limonene). Limonene increased ERK1/2 activation by 30%, while myrcene decreased it by 26%, relative to IL-1β-treated cells. None of the compounds tested was able to increase the expression of cartilage matrix-specific genes (collagen II and aggrecan), but both compounds prevented the increased expression of the non-cartilage specific, collagen I, induced by IL-1β. These data show that myrcene has significant anti-inflammatory and anti-catabolic effects in human chondrocytes and, thus, its ability to halt or, at least, slow down cartilage destruction and osteoarthritis progression warrants further investigation. | por |
| dc.identifier.citation | Eur J Pharmacol. 2015 Jan 23. pii: S0014-2999(15)00041-2. | por |
| dc.identifier.uri | http://hdl.handle.net/10400.4/1789 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.subject | Osteoartrite | por |
| dc.subject | Produtos Biológicos | por |
| dc.title | Evaluation of the anti-inflammatory, anti-catabolic and pro-anabolic effects of E-caryophyllene, myrcene and limonene in a cell model of osteoarthritis | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |