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EGFR/erB-1, HER2/erB-2, CK7, LP34, Ki67 and P53 expression in preneoplastic lesions of bronchial epithelium: an immunohistochemical and genetic study

dc.contributor.authorSousa, V
dc.contributor.authorEspírito Santo, J
dc.contributor.authorSilva, M
dc.contributor.authorCabral, T
dc.contributor.authorAlarcão, AM
dc.contributor.authorGomes, A
dc.contributor.authorCouceiro, P
dc.contributor.authorCarvalho, L
dc.date.accessioned2017-08-25T11:12:35Z
dc.date.available2017-08-25T11:12:35Z
dc.date.issued2011-05
dc.description.abstractA prognostic interpretation of preneoplastic lesions would have impact in bronchial carcinoma early diagnosis and through the study of Erb-B family receptors as they have an important role in lung carcinogenesis. The existence of drugs as tyrosine kinase inhibitors stressed the importance of studying gene alterations for selected chemoprevention schemes and characterization of carcinogenesis. Bronchial preneoplastic lesions were characterized by immunohistochemistry using the antibodies LP34 (high weigh molecular cytokeratin), CK7, chromogranin A, Ki67, p53, C-erbB-2 and EGFR. HER2 and EGFR gene copy number was also evaluated by fluorescent in situ hybridization in those lesions. The expected results defined the origin cell for basal cell hyperplasia and squamous metaplasia as adaptative lesions and dysplasia. By known experiences and published data, beyond the stem cell, the spectral evolution of bronchial preneoplastic lesions was demonstrated by characterizing basal cells (LP34) and their neoplastic potentiality. Dysplasias showed a higher expression of EGFR, Ki67 and p53 with a stepwise increase with the gravity of the respective grading. C-erbB-2 immunohistochemical overexpression was a rare event in preneoplastic lesions. Polysomy was the main mechanism for EGFR and HER2/neu higher gene copy number and together with increased proliferation index (Ki67) will account to preview bronchial carcinogenesis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVirchows Arch. 2011 May;458(5):571-81.pt_PT
dc.identifier.doi10.1007/s00428-011-1062-5pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.4/2068
dc.language.isoengpt_PT
dc.subjectQueratina-7pt_PT
dc.subjectBrônquiospt_PT
dc.subjectAntigénio ki-67pt_PT
dc.subjectReceptor do Factor de Crescimento Epidérmicopt_PT
dc.subjectProteína Supressora de Tumor p53pt_PT
dc.subjectReceptor ErbB-2pt_PT
dc.titleEGFR/erB-1, HER2/erB-2, CK7, LP34, Ki67 and P53 expression in preneoplastic lesions of bronchial epithelium: an immunohistochemical and genetic studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage581pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage571-81pt_PT
oaire.citation.volume458pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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