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Molecular and cytogenetic characterization of expanded B-cell clones from multiclonal versus monoclonal B-cell chronic lymphoproliferative disorders.

2014Journal article Open access
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dc.contributor.authorHenriques, A
dc.contributor.authorRodriguez-Caballero, A
dc.contributor.authorCriado, I
dc.contributor.authorLangerak, AW
dc.contributor.authorNieto, WG
dc.contributor.authorLecrevisse, Q
dc.contributor.authorGonzáles, M
dc.contributor.authorCortesão, E
dc.contributor.authorPaiva, A
dc.contributor.authorAlmeida, J
dc.contributor.authorOrfão, A
dc.date.accessioned2014-02-28T11:57:54Z
dc.date.available2014-02-28T11:57:54Z
dc.date.issued2014
dc.description.abstractChronic antigen-stimulation has been recurrently involved in the earlier stages of monoclonal B-cell lymphocytosis, chronic lymphocytic leukemia and other B-cell chronic lymphoproliferative disorders. Among these individuals, expansion of ≥2 B-cell clones has been frequently reported; potentially, such coexisting clones have a greater probability of interaction with common immunological determinants. Here, we comparatively analyzed the B-cell receptor repertoire and the molecular profile, as well as the phenotypic, cytogenetic and hematological features of 228 chronic lymphocytic leukemia-like and non-chronic lymphocytic leukemia-like clones between multiclonal (n=85 clones from 41 cases) versus monoclonal (n=143 clones) monoclonal B-cell lymphocytosis, chronic lymphocytic leukemia and other B-cell chronic lymphoproliferative disorders. The B-cell receptor of B-cell clones from multiclonal cases showed a slightly higher degree of HCDR3 homology than B-cell clones from monoclonal cases, in association with unique hematological (e.g. lower B-lymphocyte counts) and cytogenetic (e.g. lower frequency of cytogenetically altered clones) features usually related to earlier stages of the disease. Moreover, a subgroup of coexisting B-cell clones from individual multiclonal cases which were found to be phylogenetically related, showed unique molecular and cytogenetic features: they more frequently shared IGHV3 gene usage, shorter HCDR3 sequences with a greater proportion of IGHV mutations and del(13q14.3), than other unrelated B-cell clones. These results would support the antigen-driven nature of such multiclonal B-cell expansions, with potential involvement of multiple antigens/epitopes.por
dc.identifier.citationHaematologica. 2014 Jan 31. [Epub ahead of print]por
dc.identifier.urihttp://hdl.handle.net/10400.4/1645
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherEuropean Hematology Associationpor
dc.subjectLinfócitos Bpor
dc.subjectDoenças Linfoproliferativaspor
dc.titleMolecular and cytogenetic characterization of expanded B-cell clones from multiclonal versus monoclonal B-cell chronic lymphoproliferative disorders.por
dc.typejournal article
dspace.entity.typePublication
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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