Publication
Hepatic UDP-glucose 13C isotopomers from [U-13C]glucose: a simple analysis by 13C NMR of urinary menthol glucuronide
| dc.contributor.author | Antunes, MJ | |
| dc.contributor.author | Biernat, M | |
| dc.contributor.author | Sola, E | |
| dc.contributor.author | Oliveira, L | |
| dc.contributor.author | Prieto, D | |
| dc.contributor.author | Franco, F | |
| dc.contributor.author | Providência, LA | |
| dc.date.accessioned | 2009-05-20T14:57:26Z | |
| dc.date.available | 2009-05-20T14:57:26Z | |
| dc.date.issued | 2006 | |
| dc.description.abstract | Menthol glucuronide was isolated from the urine of a healthy 70-kg female subject following ingestion of 400 mg of peppermint oil and 6 g of 99% [U-(13)C]glucose. Glucuronide (13)C-excess enrichment levels were 4-6% and thus provided high signal-to-noise ratios (SNRs) for confident assignment of (13)C-(13)C spin-coupled multiplet components within each (13)C resonance by (13)C NMR. The [U-(13)C]glucuronide isotopomer derived via direct pathway conversion of [U-(13)C]glucose to [U-(13)C]UDP-glucose was resolved from [1,2,3-(13)C(3)]- and [1,2-(13)C(2)]glucuronide isotopomers derived via Cori cycle or indirect pathway metabolism of [U-(13)C]glucose. In a second study, a group of four overnight-fasted patients (63 +/- 10 kg) with severe heart failure were given peppermint oil and infused with [U-(13)C]glucose for 4 hr (14 mg/kg prime, 0.12 mg/kg/min constant infusion) resulting in a steady-state plasma [U-(13)C]glucose enrichment of 4.6% +/- 0.6%. Menthol glucuronide was harvested and glucuronide (13)C-isotopomers were analyzed by (13)C NMR. [U-(13)C]glucuronide enrichment was 0.6% +/- 0.1%, and the sum of [1,2,3-(13)C(3)] and [1,2-(13)C(2)]glucuronide enrichments was 0.9% +/- 0.2%. From these data, flux of plasma glucose to hepatic UDPG was estimated to be 15% +/- 4% that of endogenous glucose production (EGP), and the Cori cycle accounted for at least 32% +/- 10% of GP. | en |
| dc.identifier.citation | Magn Reson Med. 2006 Nov;56(5):1121-5 | en |
| dc.identifier.uri | http://hdl.handle.net/10400.4/506 | |
| dc.language.iso | eng | en |
| dc.rights.uri | openAccess | en |
| dc.subject | Espectroscopia de Ressonância Magnética | en |
| dc.subject | Fígado | en |
| dc.subject | Uridina Difosfato Glucose | en |
| dc.title | Hepatic UDP-glucose 13C isotopomers from [U-13C]glucose: a simple analysis by 13C NMR of urinary menthol glucuronide | en |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| rcaap.type | article | en |