Publication
Integration of [U-13C]glucose and 2H2O for quantification of hepatic glucose production and gluconeogenesis
| dc.contributor.author | Perdigoto, R | |
| dc.contributor.author | Rodrigues, TB | |
| dc.contributor.author | Furtado, AL | |
| dc.contributor.author | Porto, A | |
| dc.contributor.author | Geraldes, CF | |
| dc.contributor.author | Jones, JG | |
| dc.date.accessioned | 2008-12-15T16:47:56Z | |
| dc.date.available | 2008-12-15T16:47:56Z | |
| dc.date.issued | 2003 | |
| dc.description.abstract | Glucose metabolism in five healthy subjects fasted for 16 h was measured with a combination of [U-13C]glucose and 2H2O tracers. Phenylbutyric acid was also provided to sample hepatic glutamine for the presence of 13C-isotopomers derived from the incorporation of [U-13C]glucose products into the hepatic Krebs cycle. Glucose production (GP) was quantified by 13C NMR analysis of the monoacetone derivative of plasma glucose following a primed infusion of [U-13C]glucose and provided reasonable estimates (1.90 +/- 0.19 mg/kg/min with a range of 1.60-2.15 mg/kg/min). The same derivative yielded measurements of plasma glucose 2H-enrichment from 2H2O by 2H NMR from which the contribution of glycogenolytic and gluconeogenic fluxes to GP was obtained (0.87 +/- 0.14 and 1.03 +/- 0.10 mg/kg/min, respectively). Hepatic glutamine 13C-isotopomers representing multiply-enriched oxaloacetate and [U-13C]acetyl-CoA were identified as multiplets in the 13C NMR signals of the glutamine moiety of urinary phenylacetylglutamine, demonstrating entry of the [U-13C]glucose tracer into both oxidative and anaplerotic pathways of the hepatic Krebs cycle. These isotopomers contributed 0.1-0.2% excess enrichment to carbons 2 and 3 and approximately 0.05% to carbon 4 of glutamine. | en |
| dc.identifier.citation | NMR Biomed. 2003 Jun;16(4):189-98 | en |
| dc.identifier.uri | http://hdl.handle.net/10400.4/353 | |
| dc.language.iso | eng | en |
| dc.publisher | Wiley Interscience | en |
| dc.subject | Fígado | en |
| dc.subject | Gluconeogénese | en |
| dc.title | Integration of [U-13C]glucose and 2H2O for quantification of hepatic glucose production and gluconeogenesis | en |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | |
| rcaap.type | article | en |