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Integration of [U-13C]glucose and 2H2O for quantification of hepatic glucose production and gluconeogenesis

2003Journal article Open access
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dc.contributor.authorPerdigoto, R
dc.contributor.authorRodrigues, TB
dc.contributor.authorFurtado, AL
dc.contributor.authorPorto, A
dc.contributor.authorGeraldes, CF
dc.contributor.authorJones, JG
dc.date.accessioned2009-08-21T14:56:58Z
dc.date.available2009-08-21T14:56:58Z
dc.date.issued2003
dc.description.abstractGlucose metabolism in five healthy subjects fasted for 16 h was measured with a combination of [U-13C]glucose and 2H2O tracers. Phenylbutyric acid was also provided to sample hepatic glutamine for the presence of 13C-isotopomers derived from the incorporation of [U-13C]glucose products into the hepatic Krebs cycle. Glucose production (GP) was quantified by 13C NMR analysis of the monoacetone derivative of plasma glucose following a primed infusion of [U-13C]glucose and provided reasonable estimates (1.90 ± 0.19 mg/kg/min with a range of 1.60-2.15 mg/kg/min). The same derivative yielded measurements of plasma glucose 2H-enrichment from 2H2O by 2H NMR from which the contribution of glycogenolytic and gluconeogenic fluxes to GP was obtained (0.87 ± 0.14 and 1.03 ± 0.10 mg/kg/min, respectively). Hepatic glutamine 13C-isotopomers representing multiply-enriched oxaloacetate and [U-13C]acetyl-CoA were identified as multiplets in the 13C NMR signals of the glutamine moiety of urinary phenylacetylglutamine, demonstrating entry of the [U-13C]glucose tracer into both oxidative and anaplerotic pathways of the hepatic Krebs cycle. These isotopomers contributed 0.1-0.2% excess enrichment to carbons 2 and 3 and sim0.05% to carbon 4 of glutaminept
dc.identifier.citationNMR in Biomedicine. 16:4 (2003) 189-198pt
dc.identifier.urihttp://hdl.handle.net/10400.4/552
dc.language.isoengpt
dc.publisherJohn Wiley & Sonspt
dc.rights.uriopenAccessen
dc.subjectGlicosept
dc.titleIntegration of [U-13C]glucose and 2H2O for quantification of hepatic glucose production and gluconeogenesispt
dc.typejournal article
dspace.entity.typePublication
rcaap.typearticlept

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