Publication
Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis of 2H glucuronide enrichment.
| dc.contributor.author | Delgado, TC | |
| dc.contributor.author | Barosa, C | |
| dc.contributor.author | Castro, MM | |
| dc.contributor.author | Geraldes, CF | |
| dc.contributor.author | Bastos, M | |
| dc.contributor.author | Baptista, C | |
| dc.contributor.author | Fagulha, A | |
| dc.contributor.author | Barros, L | |
| dc.contributor.author | Mota, A | |
| dc.contributor.author | Carvalheiro, M | |
| dc.contributor.author | Jones, JG | |
| dc.contributor.author | Merrit, M | |
| dc.date.accessioned | 2008-11-27T16:59:51Z | |
| dc.date.available | 2008-11-27T16:59:51Z | |
| dc.date.issued | 2008 | |
| dc.description.abstract | The contribution of gluconeogenesis to hepatic glucose production (GP) was quantified after (2)H(2)O ingestion by Bayesian analysis of the position 2 and 5 (2)H-NMR signals (H2 and H5) of monoacetone glucose (MAG) derived from urinary acetaminophen glucuronide. Six controls and 10 kidney transplant (KTx) patients with cyclosporine A (CsA) immunosuppressant therapy were studied. Seven KTx patients were lean and euglycemic (BMI = 24.3 +/- 1.0 kg/m(2); fasting glucose = 4.7 +/- 0.1 mM) while three were obese and hyperglycemic (BMI = 30.5 +/- 0.7 kg/m(2); fasting glucose = 7.1 +/- 0.5 mM). For the 16 spectra analyzed, the mean coefficient of variation for the gluconeogenesis contribution was 10% +/- 5%. This uncertainty was associated with a mean signal-to-noise ratio (SNR) of 79:1 and 45:1 for the MAG H2 and H5 signals, respectively. For control subjects, gluconeogenesis contributed 54% +/- 7% of GP as determined by the mean and standard deviation (SD) of individual Bayesian analyses. For the lean/normoglycemic KTx subjects, the gluconeogenic contribution to GP was 62% +/- 7% (P = 0.06 vs. controls), while hyperglycemic/obese KTx patients had a gluconeogenic contribution of 68% +/- 3% (P < 0.005 vs. controls). These data suggest that in KTx patients, an increased gluconeogenic contribution to GP is strongly associated with obesity and hyperglycemia | en |
| dc.identifier.citation | Magn Reson Med. 2008 Sep;60(3):517-23 | en |
| dc.identifier.uri | http://hdl.handle.net/10400.4/209 | |
| dc.language.iso | eng | en |
| dc.subject | Gluconeogénese | en |
| dc.title | Sources of hepatic glucose production by 2H2O ingestion and Bayesian analysis of 2H glucuronide enrichment. | en |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | |
| rcaap.type | article | en |