Repository logo
 
Publication

Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line

2004Journal article Open access
Simple item page
dc.contributor.authorCruz, MT
dc.contributor.authorGonçalo, Margarida
dc.contributor.authorFigueiredo, A
dc.contributor.authorCarvalho, AP
dc.contributor.authorDuarte, CB
dc.date.accessioned2010-03-10T16:36:50Z
dc.date.available2010-03-10T16:36:50Z
dc.date.issued2004
dc.description.abstractNuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO(4)) and increases the expression of the iNOS protein, as determined by immunofluorescence and Western blot analysis. The sensitizer NiSO(4) increased cytoplasmic iNOS expression by 31.9 +/- 10.3% and nitrite production, as assayed by the Griess reaction, by 27.6 +/- 9.5%. Electrophoretic mobility shift assay (EMSA), showed that 30 min of FSDC exposure to NiSO(4) activates the transcription factor NF-kB by 58.2 +/- 7.0% and 2 h of FSDC exposure to NiSO(4) activates the transcription factor AP-1 by 26.0 +/- 1.4%. Together, these results indicate that NiSO(4) activates the NF-kB and AP-1 pathways and induces iNOS expression in skin dendritic cells.pt
dc.identifier.citationExp Dermatol. 2004 Jan;13(1):18-26.pt
dc.identifier.urihttp://hdl.handle.net/10400.4/764
dc.language.isoengpt
dc.publisherBlackwell Munksgaardpt
dc.subjectCélulas Dendríticaspt
dc.subjectNF-kappa Bpt
dc.subjectPelept
dc.subjectSintetase de Óxido Nítricopt
dc.subjectNíquelpt
dc.titleContact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell linept
dc.typejournal article
dspace.entity.typePublication
rcaap.rightsopenAccesspt
rcaap.typearticlept

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
NFkB e AP-1 activated by The contact sensitizer Ni -Experimental Dermatology.pdf
Size:
880.61 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.8 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DER - Artigos