Publication
Involvement of JAK2 and MAPK on type II nitric oxide synthase expression in skin-derived dendritic cells
| dc.contributor.author | Cruz, MT | |
| dc.contributor.author | Duarte, CB | |
| dc.contributor.author | Gonçalo, Margarida | |
| dc.contributor.author | Carvalho, AP | |
| dc.contributor.author | Lopes, MC | |
| dc.date.accessioned | 2010-03-10T12:13:41Z | |
| dc.date.available | 2010-03-10T12:13:41Z | |
| dc.date.issued | 1999 | |
| dc.description.abstract | In this report, we demonstrate that a fetal mouse skin-derived dendritic cell line produces nitric oxide (NO) in response to the endotoxin [lipopolysaccharide (LPS)] and to cytokines [tumor necrosis factor-alpha (TNF-alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF)]. Expression of the inducible isoform of NO synthase (iNOS) was confirmed by immunofluorescence with an antibody against iNOS. The tyrosine kinase inhibitor genistein decreased LPS- and GM-CSF-induced nitrite (NO(-2)) production. The effect of LPS and cytokines on NO(-2) production was inhibited by the Janus kinase 2 (JAK2) inhibitor tyrphostin B42. The p38 mitogen-activated protein kinase (p38 MAPK) inhibitor SB-203580 also reduced the NO(-2) production evoked by LPS, TNF-alpha, or GM-CSF, but it was not as effective as tyrphostin B42. Inhibition of MAPK kinase with PD-098059 also slightly reduced the effect of TNF-alpha or GM-CSF on NO(-2) production. Immunocytochemistry studies revealed that the transcription factor nuclear factor-kappaB was translocated from the cytoplasm into the nuclei of fetal skin-derived dendritic cells (FSDC) stimulated with LPS, and this translocation was inhibited by tyrphostin B42. Our results show that JAK2 plays a major role in the induction of iNOS in FSDC. | pt |
| dc.identifier.citation | Am J Physiol. 1999 Dec;277(6 Pt 1):C1050-7. | pt |
| dc.identifier.uri | http://hdl.handle.net/10400.4/744 | |
| dc.language.iso | eng | pt |
| dc.publisher | American Physiological Society | pt |
| dc.subject | Células Dendríticas | pt |
| dc.subject | Proteínas Quinases Activadas por Mitógeno | pt |
| dc.subject | Pele | pt |
| dc.subject | Síntese do Óxido Nítrico | pt |
| dc.subject | Proteína Tirosina Quinases | pt |
| dc.subject | Proteínas Proto-Oncogénicas | pt |
| dc.title | Involvement of JAK2 and MAPK on type II nitric oxide synthase expression in skin-derived dendritic cells | pt |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | pt |
| rcaap.type | article | pt |