Publication
FoxP3, GATA-3 and T-bet expression in elderly asthma
| dc.contributor.author | Vale-Pereira, S | |
| dc.contributor.author | Todo-Bom, A | |
| dc.contributor.author | Geraldes, L | |
| dc.contributor.author | Schmidt-Weber, C | |
| dc.contributor.author | Akdis, CA | |
| dc.contributor.author | Mota-Pinto, A | |
| dc.date.accessioned | 2011-11-29T11:32:36Z | |
| dc.date.available | 2011-11-29T11:32:36Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | BACKGROUND: Asthma is a chronic inflammatory disorder in which Th2, Th1 and suppressive T cells (Tregs) play a role. The transcription factor FoxP3 plays a role in Treg differentiation while T-bet is important for Th1 and GATA-3 for Th2 differentiation from naïve T cells. Recent data show that age-related deregulation of Treg cells is a mechanism of senescence affecting several chronic diseases. It is crucial to understand the behaviour of these cell populations in asthma for elderly patients. OBJECTIVE: To evaluate FoxP3, GATA-3 and T-bet gene expression under basal conditions and after in vitro activation in a group of elderly asthmatic compared with age-matched healthy individuals. METHODS: Thirty-two elderly asthmatics and 17 healthy elderly individuals were selected. Serum total IgE was measured, and peripheral blood mononuclear cells (PBMCs) were isolated and stimulated in vitro with anti-CD3/anti-CD28, followed by mRNA isolation. After reverse transcription, real-time quantitative PCR was performed and relative quantification was determined 2(-ΔΔCt)(2(-ΔΔCt) method). RESULTS: The mean values and standard deviation of FoxP3, GATA-3 and T-bet relative expression for control vs. asthma were 10.2±6.8 vs. 4.8±3.8, 2.4±2.9 vs. 1.7±0.9 and 3.3±2.1 vs. 2.1±1.5, respectively. Healthy individuals showed significantly higher expression of FoxP3 and T-bet; asthmatics had a lower T-bet/GATA-3 ratio, higher serum IgE and a positive significant correlation between total IgE and GATA-3 expression. CONCLUSION AND CLINICAL RELEVANCE: Elderly asthmatic patients have lower FoxP3 mRNA expression in PBMC, which can be associated with the sustained inflammatory process and with the decreased immune tolerance by Treg cells. The T-bet deficiency and the correlation of GATA-3 expression with the increase of IgE are characteristics of long-lasting asthma. Changes related to the immunosenescence process could provide an explanation for the minor differences observed between the groups. It is important to clarify persistent modifications in long-lasting asthma in the elderly and adequate future therapeutic approaches. | por |
| dc.identifier.citation | Clin Exp Allergy. 2011 Apr;41(4):490-6. | por |
| dc.identifier.uri | http://hdl.handle.net/10400.4/1174 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Blackwell Publishing | por |
| dc.subject | Asma | por |
| dc.subject | Linfócitos T Reguladores | por |
| dc.subject | Idoso | por |
| dc.title | FoxP3, GATA-3 and T-bet expression in elderly asthma | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
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