Publication
Effect of lipopolysaccharide, skin sensitizers and irritants on thioredoxin-1 expression in dendritic cells: relevance of different signalling pathways
| dc.contributor.author | Francisco, V | |
| dc.contributor.author | Neves, BM | |
| dc.contributor.author | Cruz, MT | |
| dc.contributor.author | Gonçalo, Margarida | |
| dc.contributor.author | Figueiredo, A | |
| dc.contributor.author | Duarte, CB | |
| dc.contributor.author | Lopes, MC | |
| dc.date.accessioned | 2011-02-22T10:14:45Z | |
| dc.date.available | 2011-02-22T10:14:45Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Thioredoxin-1 is a ubiquitous protein involved in phenotypical and functional changes in dendritic cells (DC). We investigated the effect of lipopolysaccharide (LPS), skin sensitizers, and irritants on thioredoxin-1 by Western blot and immunofluorescence and on mRNA by real-time PCR. As DC models, we used a skin DC line and DC derived from human blood monocytes. We observed that all tested chemicals increased thioredoxin-1 expression, which is only transient for irritants, being the strongest effect observed for LPS (63 +/- 15%). To address the involvement of thioredoxin-1 in DC maturation, we analysed the effect of an activator of thioredoxin-1 expression, hydrogen peroxide, on CD86 expression, a marker of DC maturation. We found that hydrogen peroxide increases thioredoxin-1 and CD86 expression reinforcing thioredoxin-1 involvement in DC maturation. Because mitogen-activated protein kinases and PI3K are activated upon DC maturation, we also analysed their involvement in thioredoxin-1 modulation. We verified that LPS-induced upregulation of thioredoxin-1 expression was dependent on PI3K pathway. | por |
| dc.identifier.citation | Arch Dermatol Res. 2010 May;302(4):271-82. | por |
| dc.identifier.uri | http://hdl.handle.net/10400.4/994 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Springer | por |
| dc.subject | Antigénio CD86 | por |
| dc.subject | Células de Langerhans | por |
| dc.subject | Tioredoxinas | por |
| dc.title | Effect of lipopolysaccharide, skin sensitizers and irritants on thioredoxin-1 expression in dendritic cells: relevance of different signalling pathways | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |