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Endometrial Cancer Spheres Show Cancer Stem Cells Phenotype and Preference for Oxidative Metabolism

2018-11-29Journal article Open access
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dc.contributor.authorCarvalho, MJ
dc.contributor.authorLaranjo, M
dc.contributor.authorAbrantes, AM
dc.contributor.authorCasalta-Lopes, J
dc.contributor.authorSarmento-Santos, D
dc.contributor.authorCosta, T
dc.contributor.authorSerambeque, B
dc.contributor.authorAlmeida, N
dc.contributor.authorGonçalves, T
dc.contributor.authorMamede, C
dc.contributor.authorEncarnação, J
dc.contributor.authorOliveira, R
dc.contributor.authorPaiva, A
dc.contributor.authorde Carvalho, R
dc.contributor.authorBotelho, F
dc.contributor.authorOliveira, C
dc.date.accessioned2019-07-02T14:57:21Z
dc.date.available2019-07-02T14:57:21Z
dc.date.issued2018-11-29
dc.description.abstractThis study aimed to characterize endometrial cancer regarding cancer stem cells (CSC) markers, regulatory and differentiation pathways, tumorigenicity and glucose metabolism. Endometrial cancer cell line ECC1 was submitted to sphere forming protocols. The first spheres generation (ES1) was cultured in adherent conditions (G1). This procedure was repeated and was obtained generations of spheres (ES1, ES2 and ES3) and spheres-derived cells in adherent conditions (G1, G2 and G3). Populations were characterized regarding CD133, CD24, CD44, aldehyde dehydrogenase (ALDH), hormonal receptors, HER2, P53 and β-catenin, fluorine-18 fluorodeoxyglucose ([18F]FDG) uptake and metabolism by NMR spectroscopy. An heterotopic model evaluated differential tumor growth. The spheres self-renewal was higher in ES3. The putative CSC markers CD133, CD44 and ALDH expression were higher in spheres. The expression of estrogen receptor (ER)α and P53 decreased in spheres, ERβ and progesterone receptor had no significant changes and β-catenin showed a tendency to increase. There was a higher 18F-FDG uptake in spheres, which also showed a lower lactate production and an oxidative cytosol status. The tumorigenesis in vivo showed an earlier growth of tumours derived from ES3. Endometrial spheres presented self-renewal and differentiation capacity, expressed CSC markers and an undifferentiated phenotype, showing preference for oxidative metabolism.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPathol Oncol Res. 2018 Nov 29. doi: 10.1007/s12253-018-0535-0pt_PT
dc.identifier.doi10.1007/s12253-018-0535-0pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.4/2239
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectNeoplasias do Endométriopt_PT
dc.subjectCélulas Estaminaispt_PT
dc.subjectStress Oxidativopt_PT
dc.titleEndometrial Cancer Spheres Show Cancer Stem Cells Phenotype and Preference for Oxidative Metabolismpt_PT
dc.typejournal article
dspace.entity.typePublication
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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