Repository logo
 
Publication

In vivo selective and distant killing of cancer cells using adenovirus-mediated decorin gene transfer

2003-03Journal article Open access
Simple item page
dc.contributor.authorTralhão, JG
dc.contributor.authorSchaefer, L
dc.contributor.authorMicegova, M
dc.contributor.authorEvaristo, C
dc.contributor.authorSchönherr, E
dc.contributor.authorKayal, S
dc.contributor.authorVeiga-Fernandes, H
dc.contributor.authorDanel, C
dc.contributor.authorIozzo, RV
dc.contributor.authorKresse, H
dc.contributor.authorLemarchand, P
dc.date.accessioned2018-11-28T15:15:42Z
dc.date.available2018-11-28T15:15:42Z
dc.date.issued2003-03
dc.description.abstractDecorin is a well-known, ubiquitous proteoglycan that is a normal component of the ECM. Upon transgenic expression of decorin, tumor cells with diverse histogenetic background overexpress p21WAF1, a potent inhibitor of cyclin-dependent kinase activity, become arrested in G1, and fail to generate tumors in immunocompromised animals. Because decorin is a secreted protein, it has been recently suggested that decorin could act as an autocrine and paracrine regulator of tumor growth. Here, we demonstrate that adenovirus (Ad)-mediated transfer and expression of human decorin cDNA induced in vivo apoptosis of xenograft tumor cells in nude mice. This oncolytic activity was observed when the Ad vector encoding the decorin cDNA was injected intratumorally (i.t.) or i.v. Importantly, i.t. injection of the decorin Ad vector led to growth inhibition of the injected tumor associated with similar growth inhibition of a distant contralateral tumor, demonstrating a distant decorin antitumoral effect. Immunochemistry against human decorin and decorin quantitation in tumors confirmed that decorin migrated to the tumor distant site. Furthermore, decorin effect was specific to tumor cells, because neither apoptosis nor growth inhibition were observed in nontumoral human cells such as hepatocytes, endothelial cells, and fibroblasts, despite p21 overexpression.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFASEB J. 2003 Mar;17(3):464-6.pt_PT
dc.identifier.doi10.1096/fj.02-0534fjept_PT
dc.identifier.urihttp://hdl.handle.net/10400.4/2186
dc.language.isoengpt_PT
dc.subjectAdenoviridae/genéticapt_PT
dc.subjectTerapia Genéticapt_PT
dc.subjectNeoplasias Experimentaispt_PT
dc.subjectProteoglicanas/genéticapt_PT
dc.titleIn vivo selective and distant killing of cancer cells using adenovirus-mediated decorin gene transferpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage466pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage464-6pt_PT
oaire.citation.volume17pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
In vivo selective and distant killing of cancer cells.pdf
Size:
113.44 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIR - Artigos