Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.4/744
Título: Involvement of JAK2 and MAPK on type II nitric oxide synthase expression in skin-derived dendritic cells
Autor: Cruz, MT
Duarte, CB
Gonçalo, Margarida
Carvalho, AP
Lopes, MC
Palavras-chave: Células Dendríticas
Proteínas Quinases Activadas por Mitógeno
Pele
Síntese do Óxido Nítrico
Proteína Tirosina Quinases
Proteínas Proto-Oncogénicas
Data: 1999
Editora: American Physiological Society
Citação: Am J Physiol. 1999 Dec;277(6 Pt 1):C1050-7.
Resumo: In this report, we demonstrate that a fetal mouse skin-derived dendritic cell line produces nitric oxide (NO) in response to the endotoxin [lipopolysaccharide (LPS)] and to cytokines [tumor necrosis factor-alpha (TNF-alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF)]. Expression of the inducible isoform of NO synthase (iNOS) was confirmed by immunofluorescence with an antibody against iNOS. The tyrosine kinase inhibitor genistein decreased LPS- and GM-CSF-induced nitrite (NO(-2)) production. The effect of LPS and cytokines on NO(-2) production was inhibited by the Janus kinase 2 (JAK2) inhibitor tyrphostin B42. The p38 mitogen-activated protein kinase (p38 MAPK) inhibitor SB-203580 also reduced the NO(-2) production evoked by LPS, TNF-alpha, or GM-CSF, but it was not as effective as tyrphostin B42. Inhibition of MAPK kinase with PD-098059 also slightly reduced the effect of TNF-alpha or GM-CSF on NO(-2) production. Immunocytochemistry studies revealed that the transcription factor nuclear factor-kappaB was translocated from the cytoplasm into the nuclei of fetal skin-derived dendritic cells (FSDC) stimulated with LPS, and this translocation was inhibited by tyrphostin B42. Our results show that JAK2 plays a major role in the induction of iNOS in FSDC.
URI: http://hdl.handle.net/10400.4/744
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