Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.4/2190
Título: Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice
Autor: Sidorkiewicz, M
Jais, JP
Tralhao, JG
Morosan, S
Giannini, C
Brezillon, N
Soussan, P
Delpuech, O
Kremsdorf, D
Palavras-chave: Hepatite B Crónica
Regeneração Hepática
Análise de Sequência com Séries de Oligonucleotídeos
Transativadores
Data: 28-Jan-2008
Citação: World J Gastroenterol. 2008 Jan 28;14(4):574-81.
Resumo: AIM: To analyze the modulation of gene expression profile associated with inhibition of liver regeneration in hepatitis B X (HBx)-expressing transgenic mice. METHODS: Microarray technology was performed on liver tissue obtained from 4 control (LacZ) and 4 transgenic mice (HBx-LacZ), 48 h after partial hepatectomy. The significance of the normalized log-ratios was assessed for each gene, using robust t-tests under an empirical Bayes approach. Microarray hybridization data was verified on selected genes by quantitative PCR. RESULTS: The comparison of gene expression patterns showed a consistent modulation of the expression of 26 genes, most of which are implicated in liver regeneration. Up-regulated genes included DNA repair proteins (Rad-52, MSH6) and transmembrane proteins (syndecan 4, tetraspanin), while down-regulated genes were connected to the regulation of transcription (histone deacetylase, Zfp90, MyoD1) and were involved in the cholesterol metabolic pathway and isoprenoid biosynthesis (farnesyl diphosphate synthase, Cyp7b1, geranylgeranyl diphosphate synthase, SAA3). CONCLUSION: Our results provide a novel insight into the biological activities of HBx, implicated in the inhibition of liver regeneration.
Peer review: yes
URI: http://hdl.handle.net/10400.4/2190
Aparece nas colecções:CIR - Artigos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
World J Gastroenterol.pdf715,08 kBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.